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| 產地 | 中國/美國 |
| 品牌 | KALANG/進口品牌 |
| 貨號 | kl-C027Hu01 |
| 保存條件 | -20℃ |
| 英文名稱 | Recombinant O-6-Methylguanine DNA Methyltransferase (MGMT) |
| CAS編號 | |
| 保質期 | 12個月個月 |
O-6-甲基鳥嘌呤DNA甲基轉移酶(MGMT)重組蛋白
Recombinant O-6-Methylguanine DNA Methyltransferase (MGMT)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 25.3kDa |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1μg (determined by the LAL method) |
| Residues | Met1~Asn207 with two N-terminal Tags, His-tag and T7-tag |
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
O-6-甲基鳥嘌呤DNA甲基轉移酶(MGMT)重組蛋白SEQUENCES
The sequence of the target protein is listed below.
MDKDCEMKRT TLDSPLGKLE LSGCEQGLHE IKLLGKGTSA ADAVEVPAPA AVLGGPEPLM QCTAWLNAYF HQPEAIEEFP VPALHHPVFQ QESFTRQVLW KLLKVVKFGE VISYQQLAAL AGNPKAARAV GGAMRGNPVP ILIPCHRVVC SSGAVGNYSG GLAVKEWLLA HEGHRLGKPG LGGSSGLAGA WLKGAGATSG SPPAGRN
USAGE
Reconstitute in sterile ddH2O.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
O-6-甲基鳥嘌呤DNA甲基轉移酶(MGMT)重組蛋白Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
O-6-甲基鳥嘌呤DNA甲基轉移酶(MGMT)重組蛋白About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
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