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上海康朗生物科技有限公司
腎母細胞瘤蛋白1(WT1)重組蛋白
  • 品牌:KALANG/進口品牌
  • 產地:中國/美國
  • 貨號:kl-F116Hu01
  • 發布日期: 2016-03-08
  • 更新日期: 2024-12-25
產品詳請
產地 中國/美國
品牌 KALANG/進口品牌
貨號 kl-F116Hu01
保存條件 -20℃
英文名稱 Recombinant Wilms Tumor Protein (WT1)
CAS編號
保質期 12個月個月

腎母細胞瘤蛋白1(WT1)重組蛋白

Recombinant Wilms Tumor Protein (WT1)

FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!

Organism speciesHomo sapiens (Human)
Product No.
SourceProkaryotic expression
HostE.coli
Purity> 90%
UOM50ug
Predicted Molecular Mass46.5kDa
Concentrationn/a
ApplicationsSDS-PAGE; WB; ELISA; IP.
Endotoxin Level<1.0EU per 1μg (determined by the LAL method)
ResiduesLeu310~Leu449 with two N-terminal Tags, His-tag and T7-tag

FormulationSupplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.

腎母細胞瘤蛋白1(WT1)重組蛋白SEQUENCES

The sequence of the target protein is listed below.
L VRSASETSEK RPFMCAYPGC NKRYFKLSHL QMHSRKHTGE KPYQCDFKDC ERRFSRSDQL KRHQRRHTGV KPFQCKTCQR KFSRSDHLKT HTRTHTGKTS EKPFSCRWPS CQKKFARSDE LVRHHNMHQR NMTKLQLAL

USAGE

Reconstitute in sterile ddH2O.

STORAGE AND STABILITY

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.

腎母細胞瘤蛋白1(WT1)重組蛋白Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

腎母細胞瘤蛋白1(WT1)重組蛋白About the MARKER (complimentary)

Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.

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